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. 2022 Mar 24;11:e77610. doi: 10.7554/eLife.77610

Figure 2. Increased heterogeneity of cell types was found in both TA and EDL of Acvr1b:Tgfbr1 CKO animals.

Figure 2.

(A) Regions with spontaneously regenerating myofibres (circled by yellow dash lines) with central nuclei (indicated by arrows) were particularly present in low oxidative region of TA and EDL of Acvr1b:Tgfbr1 CKO animals. (B) Increased number of Pax7+ cells per myofibre was found in TA of Acvr1b:Tgfbr1 CKO mice. (C) IF staining of F4/80 (green) showed an increased number of macrophages (indicated by arrows) in TA muscle per mm2 CSA of Acvr1b:Tgfbr1 CKO mice compared to control. Macrophages (image with higher magnification on the left corner) were mainly located around myofibres with central nuclei. Scale bar = 100 µm. N = 5–8 mice. Results are presented as mean + SEM. *. p < 0.05. Significant differences between individual groups are indicated by lines with a *. Single * indicates significant difference compared to all other groups at the same time point.

Figure 2—source data 1. Quantification of number of regenerating myofibres, satellite cells and macrophages in absence of injury.