. 2022 Mar 24;11:e77610. doi: 10.7554/eLife.77610

Key resources table.

Reagent type (species) or resource	Designation	Source or reference	Identifiers	Additional information
Strain, strain background (C57BL/6, males)	C57BL/6	PMID:22564549	# 025750	Jackson Laboratory, Bar Harbor, ME, USA
Genetic reagent (Transfected construct (Mus musculus))	HSA-Cre	PMID:22564549	# 025750	Jackson Laboratory, Bar Harbor, ME, USA
Genetic reagent (Transfected construct (Mus musculus))	Acvr1b^fl/fl	PMID:23109354		Cancer Research Center of Lyon, French Institute of Health and Medical Research
Genetic reagent (Transfected construct (Mus musculus))	Tgfbr1^fl/fl	PMID:11285230		Leiden University Medical Center
Antibody	Anti-phospho-Smad2 (Ser465/467) (138D4) (Rabbit monoclonal)	Cell Signaling Technology	Cat# 3108, RRID:AB_490941	WB (1:500)
Antibody	Anti-phospho-Smad3 (Ser423/425) (C25A9) (Rabbit monoclonal)	Cell Signaling Technology	Cat# 9520, RRID:AB_10203253	WB (1:500)
Antibody	Anti-Smad2/3 (Mouse monoclonal)	Cell Signaling Technology	Cat# 610843, RRID: AB_398162	WB (1:500)
Antibody	Anti-phospho-AKT (Ser473) (Rabbit monoclonal)	Cell Signaling Technology	Cat# 9271, RRID:AB 329825	WB (1:1000)
Antibody	Anti-AKT (pan) (C67E7) (Rabbit monoclonal)	Cell Signaling Technology	Cat# 4691, RRID:AB_915783	WB (1:2000)
Antibody	Anti-phospho-p70S6 Kinase (Thr389) (108D2) (Rabbit monoclonal)	Cell Signaling Technology	Cat# 9234, RRID:AB_2269803	WB (1:2000)
Antibody	Anti-p70S6 Kinase (49D7) (Rabbit monoclonal)	Cell Signaling Technology	Cat# 2708, RRID:AB_390722	WB (1:2000)
Antibody	Anti-Pan-Actin (Rabbit polyclonal)	Cell Signaling Technology	Cat# 4968, RRID:AB_2313904	WB (1:2000)
Antibody	Anti-Rabbit Immunoglobulins/HRP (Goat polyclonal)	Dako, Agilent	Cat# P0448, RRID:AB_2617138	WB (1:2000)
Antibody	Anti-Mouse IgG (H + L), HRP (Rabbit polyclonal)	Thermo Fisher Scientific	Cat# 31457, RRID:AB_228439	WB (1:2000)
Antibody	Anti-MHC-I (Mouse monoclonal)	DSHB	Cat# BA-D5, RRID:AB_2235587	IF (1 µg/mL)
Antibody	Anti-MHC-IIA (Mouse monoclonal)	DSHB	Cat# SC-71, RRID:AB_2147165	IF (10 µg/mL)
Antibody	Anti-MHC-IIB (Mouse monoclonal)	DSHB	Cat# BF-F3, RRID:AB_2266724	IF (1 µg/mL)
Antibody	Anti-MHC-IIX (Mouse monoclonal)	DSHB	Cat# 6H1, RRID:AB_1157897	IF (1 µg/mL)
Antibody	Anti-embryonic myosin heavy chain (eMyHc) (Mouse monoclonal)	DSHB	Cat# F1.652, RRID:AB_528358	IF (20 µg/mL)
Antibody	Anti-Pax7 (Mouse monoclonal)	DSHB	Cat# PAX7, RRID:AB_2299243	IF (4 µg/mL)
Antibody	Anti-F4/80 (D4C8V) XP (Rabbit monoclonal)	Cell Signaling Technology	Cat# 30325, RRID:AB_2798990	IF (0.5 µg/mL)
Antibody	Anti-Myogenin (Mouse monoclonal)	DSHB	Cat# f5d, RRID:AB_2146602	IF (0.6 µg/mL)
Antibody	Anti-Ki67 (Rabbit monoclonal)	Cell Signaling Technology	Cat# 11882, RRID:AB_2687824	IF (1:200)
Antibody	Anti-Mouse Alexa Fluor 647 IgG_2b (Goat polyclonal)	Thermo Scientific	Cat# A-21242, RRID:AB_2535811	IF (5 µg/mL)
Antibody	Anti-Mouse Alexa Fluor 488 IgG₁ (Goat polyclonal)	Thermo Scientific	Cat# A-21121, RRID:AB_2535764	IF (5 µg/mL)
Antibody	Anti-Mouse Alexa Fluor 647 IgM (Goat polyclonal)	Thermo Scientific	Cat# A21238, RRID:AB_1500930	IF (5 µg/mL)
Antibody	Anti-Mouse Alexa Fluor 488 IgM (Goat polyclonal)	Thermo Scientific	Cat# A-21042, RRID:AB_141357	IF (5 µg/mL)
Antibody	Anti-Mouse Alexa Fluor 488 IgG (H + L) (Goat polyclonal)	Thermo Scientific	Cat# A-11029, RRID:AB_2534088	IF (4–5 µg/mL)
Antibody	Anti-rabbit IgG (H + L), F(ab')2 Fragment Alexa Fluor 488 Conjugate (Goat polyclonal)	Cell Signaling Technology	Cat# 4412, RRID:AB_1904025	IF (5 µg/mL)
Sequence-based reagent	Rps13-F	This paper	PCR primers	CACGTGGCTGAAGTTGACG
Sequence-based reagent	Rps13-R	This paper	PCR primers	CAGGATTACACCTATCTGGGAGG
Sequence-based reagent	Rpl27-F	This paper	PCR primers	AGCCGTCATCGTGAAGAAC
Sequence-based reagent	Rpl27-R	This paper	PCR primers	GGGGATAGCGGTCAATTCC
Sequence-based reagent	Tgfbr1-F	This paper	PCR primers	CCTCGAGACAGGCCATTTGT
Sequence-based reagent	Tgfbr1-F	This paper	PCR primers	AGACGAAGCAGACTGGACCA
Sequence-based reagent	Acvr1b-F	This paper	PCR primers	TGCTGCGCCATGAAAACATC
Sequence-based reagent	Acvr1b-F	This paper	PCR primers	TGCCCACAATCTCCATATGCA
Sequence-based reagent	Tgfb1-F	This paper	PCR primers	GCTGACCCCCACTGATACG
Sequence-based reagent	Tgfb1-R	This paper	PCR primers	CCTGTATTCCGTCTCCTTGGTT
Sequence-based reagent	Mstn-F	This paper	PCR primers	GAGAATGGCCATGATCTTGCTG
Sequence-based reagent	Mstn-R	This paper	PCR primers	CTTCTAAAAAGGGATTCAGCCCATC
Sequence-based reagent	Igf1ea-F	This paper	PCR primers	GTGTTGCTTCCGGAGCTGTG
Sequence-based reagent	Igf1ea-R	This paper	PCR primers	CAATGTACTTCCTTCTGAGTC
Sequence-based reagent	Hgf-F	This paper	PCR primers	GATTATTGCCCTATTTCCCGTTGTG
Sequence-based reagent	Hgf-R	This paper	PCR primers	TGGCACAGGATATTACAGGATGG
Sequence-based reagent	Igf1ec-F	This paper	PCR primers	GGAGAAGGAAAGGAAGTACATTTG
Sequence-based reagent	Igf1ec-R	This paper	PCR primers	CCTGCTCCGTGGGAGGCT
Sequence-based reagent	Vegfa-F	This paper	PCR primers	CTGTAACGATGAAGCCCTGGAGTG
Sequence-based reagent	Vegfa-R	This paper	PCR primers	GGTGAGGTTTGATCCGCATGATCT
Sequence-based reagent	Pax7-F	This paper	PCR primers	TCCATCAAGCCAGGAGACA
Sequence-based reagent	Pax7-R	This paper	PCR primers	AGGAAGAAGTCCCACACAG
Sequence-based reagent	Myod-F	This paper	PCR primers	CATCCAGCCCGCTCCAAC
Sequence-based reagent	Myod-R	This paper	PCR primers	GGGCCGCTGTAATCCATCATGCC
Sequence-based reagent	Myog-F	This paper	PCR primers	CCCAACCCAGGAGATCATTT
Sequence-based reagent	Myog-R	This paper	PCR primers	GTCTGGGAAGGCAACAGACA
Sequence-based reagent	Myh3-F	This paper	PCR primers	CGCAGAATCGCAAGTCAATA
Sequence-based reagent	Myh3-R	This paper	PCR primers	CAGGAGGTCTTGCTCACTCC
Sequence-based reagent	Id1-F	This paper	PCR primers	ACCCTGAACGGCGAGATCA
Sequence-based reagent	Id1-R	This paper	PCR primers	TCGTCGGCTGGAACACAT
Sequence-based reagent	Fgf2-F	This paper	PCR primers	AAGCGGCTCTACTGCAAGAA
Sequence-based reagent	Fgf2-R	This paper	PCR primers	GTAACACACTTAGAAGCCAGCAG
Sequence-based reagent	Ccn2-F	This paper	PCR primers	CCACCCGAGTTACCAATGAC
Sequence-based reagent	Ccn2-R	This paper	PCR primers	GCTTGGCGATTTTAGGTGTC
Sequence-based reagent	Col1a1-F	This paper	PCR primers	ATGTTCAGCTTTGTGGACCT
Sequence-based reagent	Col1a1-R	This paper	PCR primers	CAGCTGACTTCAGGGATGT
Sequence-based reagent	Col3a1-F	This paper	PCR primers	AAGGACATCGAGGATTCCCTG
Sequence-based reagent	Col3a1-R	This paper	PCR primers	AGCCCTCAGATCCTCTTTCAC
Sequence-based reagent	Cd68-F	This paper	PCR primers	TCCCAACAAAACCAAGGTCCA
Sequence-based reagent	Cd68-R	This paper	PCR primers	GGCTCTGATGTAGGTCCTGTTT
Sequence-based reagent	Cd163-F	This paper	PCR primers	CGGCCCCATGAAGAGGTATC
Sequence-based reagent	Cd163-R	This paper	PCR primers	GACGGTTGACCCAGTTGTTG
Sequence-based reagent	Il1b-F	This paper	PCR primers	GCCACCTTTTGACAGTGATG
Sequence-based reagent	Il1b-R	This paper	PCR primers	CTTCTCCACAGCCACAATGA
Sequence-based reagent	Il6-F	This paper	PCR primers	GGAAATGAGAAAAGAGTTGTGC
Sequence-based reagent	Il6-R	This paper	PCR primers	GTACTCCAGAAGACCAGAGGA
Sequence-based reagent	Fbxo32-F	This paper	PCR primers	AGACTGGACTTCTCGACTGC
Sequence-based reagent	Fbxo32-R	This paper	PCR primers	TCAGCTCCAACAACAGCCTTACT
Sequence-based reagent	Trim63-F	This paper	PCR primers	CGTCCAGAGCGTGTGTCTCACTC
Sequence-based reagent	Trim63-R	This paper	PCR primers	GGGCTACCTTCCTCTCAAGTGC
Sequence-based reagent	Tcf4-F	This paper	PCR primers	GGAAAGCCCTAGCTTCGATCT
Sequence-based reagent	Tcf4-R	This paper	PCR primers	GGAGCCCACAGGAGTTGAA
Sequence-based reagent	Pdgfra-F	This paper	PCR primers	ACTTTTCACTCCGGGTATCGG
Sequence-based reagent	Pdgfra-R	This paper	PCR primers	CCCATAGCTCCTGAGACCTTC
Commercial assay or kit	RiboPure RNA Purification Kit	Thermo Fisher Scientific	AM1924
Commercial assay or kit	SuperScript VILO Mastermix	Thermo Fisher Scientific	12023679