Figure 9. Conservation of the sulfotyrosine sites in the N-terminal region of leukotoxins receptors and structural alignment of Staphylococcus aureus bicomponent pore forming toxins in the sulfotyrosine binding region (site 1).

(A) Manual sequence alignment of the sulfotyrosine motifs in the N-terminal region of the bicomponent pore forming toxin receptors. (B–J) Comparison of sulfotyrosine site 1 residues in SA bi-component leukotoxin x-ray structures. (B) LukE apo1. (C) LukD (PDB ID 6U2S, Liu et al., 2020). (D) HlgA (PDB ID 2QK7, Roblin et al., 2008). (E) HlgC (PDB ID 4P1X, Yamashita et al., 2014). (F) HlgB (PDB ID 3LKF, Olson et al., 1999). (G) LukS-PV (PDB ID 1T5R, Guillet et al., 2004). (H) LukF-PV (PDB ID 1PVL, Pédelacq et al., 1999). (I and J) LukG and lukH (PDB ID 5K59, Badarau et al., 2016).