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Schematic representation of procedure for markerless deletion of the chromosomal mexXY gene. (A) Construction of plasmid for deletion of mexXY. The striped boxes represent the mexXY-flanking regions amplified by PCR. (B) Procedure for deletion of chromosomal mexXY. The thin line represents the plasmid sequences. The thick line represents the P. aeruginosa chromosome. The straight lines at the bottom represent the size of PCR products.
