Table 2.
PEI as Co-Delivery System for Breast Cancer Therapy
| No | PEI Type | Anticancer Drugs | Gene Therapy | Combination | In vitro | In vivo | Reference |
|---|---|---|---|---|---|---|---|
| 1. | PEtOx-co-PEI | DOX | DOX-loaded PEtOx-co-PEI-b-PCL PEtOx-co-PEI complexed with PCL, and loaded with DOX |
MCF-7 | - | [32] | |
| 2. | 25 kDa | - | siEGFR1, siEGFR2, siEGFR3, siBRD41, siBRD42, siBRD43 | GALA-PEG-SS-PEI and CREKA-PEG-SS-PEI GALA- and CREKA-modified with PEG; SS; PEI (PEG-SS-PEI) |
MDA-MB-231 and HUVEC | - | [40] |
| 3. | Branched, 1.2 kDa | - | siRNA, pDNA | pDNA/siRNA/lipopolymer Lipid-grafted PEI loaded with pTRAIL and silencing BCL2L12 and SOD1 siRNA |
MCF-7, MDA-MB-231 | MDA-MB-231 xenografted mice | [57] |
| 4. | 1.3 kDa | DOX | pArg | DOX-loaded CA-PEI-pArg PMs pH-responsive PMs contain CA-PEI-pArg copolymers, loaded with DOX |
MCF-7 | - | [43] |
| 5. | Linear, 5 kDa | GA | pTRAIL | GA/pTRAIL-HA/PP Adding HA to neutralize the excess positive charge |
MDA-MB-231 and MCF-7 | Tumor-bearing nude BALB/c mice (5-week-old, 18–20 g, female) | [54] |
| 6. | Branched, 10 kDa | DOX | Bcl-xL shRNA | PLGA-DOX-Alkyl-PEI NPs/shRNA DOX encapsulated by PLGA, complexed with PEI modified with alkyl chain, and loaded with Bcl-xL shRNA. |
MCF-7 | - | [45] |
| 7. | Branched, 1 kDa | DOX | p53 | PDP/DOX/p53 Grafted lipophilic deoxycholic acid and low molecular weight (1 kDa) branched PEI to pullulan, and complexed with DOX and p53 |
MCF-7 | 6–8-week-old female BALB/C nude mice | [47] |
| 8. | Branched, 25 kDa | DOX | pORF-hTRAIL | PCL-PEI-PEG-T7/DOX/pORF-hTRAIL PEG-PEI-PCL-S-S-PCL-PEI-PEG loaded with pORF-hTRAIL and DOX |
MCF-7 | 6-week-old BALB/c female nude mice | [50] |
| 9. | Branched, 1.8 kDa | EMB | pTRAIL | EMB/TRAIL-HA/PBAE-PEI EMB and pTRAIL delivered with amphiphilic polymers PEI- PBAE. Tumor targeting mediated by HA |
MDA-MB-231 | - | [59] |
| 10. | Branched, 1.8 kDa | GemC18 | NF-κB siRNA | GemC18-containing PMs/anti NF-κB siRNA PCL-PEI-PEG functionals PMs, loaded with 4-(N)-stearoyl GemC18 and NF-κB siRNA |
AsPC-1 and MCF7 | Balb/c mice bearing 4T1 xenograft breast tumor | [60] |
| 11. | JetPEI | DOX | miR-142-3p | Jet PEI/CD44-specific-siRNA/DOX Jet PEI complexed with CD44-specific-siRNA and DOX |
MDA-MB-468 | - | [62] |
Abbreviations: Bcl-Xl, B-cell lymphoma-extra-large; BCL2L12, Bcl2-like12; CA, cholic acid; DOX, doxorubicin; EMB, embelin; GemC18, gemcitabine pro-drug; HA, hyaluronic acid; hTRAIL, human tumor necrosis factor-related apoptosis-inducing ligand; NF-κB, nuclear factor-κB; pArg, poly-L-arginine; PBAE, poly[(1,6-hexanediol)-diacrylate-β-5-hydroxyamylamine; PCL, poly(E-caprolactone); pDNA, plasmid DNA; PEG, polyethylene glycol; PEI, polyethyleneimine; PetOx-co-PEI, poly(2-ethyl-2-oxazoline)-co-poly(ethyleneimine); PLGA, poly-(lactic-co-glycolic) acid; PMs, polymeric micelles; pORF, plasmid open reading frame; pTRAIL, tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) plasmid; siRNA, small interfering RNA; SOD1, superoxide dismutase 1; SS, disulfide.