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Effect of choline treatment on apoptosis in cardiac tissue. (a) TUNEL staining; bar = 50 μm. (b) Quantitative analysis of the positive apoptotic cells (%); n = 4. (c) Western blotting bands for Bcl-2, c-caspase 3 and Bax. (d–g) Quantitative results of Bcl-2, c-caspase 3, Bax, and Bcl-2/Bax; n = 4. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, and∗∗∗∗P < 0.0001.
