Figure 5.

OCT1 positively regulates PFN2 and SNTB1. The effect of transient transfection of OCT1 on gene expression. PC3 cells were transfected with or without OCT1 expression plasmid (PC3-OCT1 and PC3) for 48 h. OCT1 expression was analyzed by Western blotting (A), and expression of each candidate gene was analyzed by qRT-PCR. *p < 0.05, **p < 0.01, Student’s t-tests. Bars, SEM (B). (C) Expression of SNTB1 and PFN2 mRNA is higher in bone metastatic prostate cancer tissues. These expression levels were analyzed using a public database in Oncomine⁴⁹. The original Western blots are presented in Supplementary Fig. S2.