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. Author manuscript; available in PMC: 2023 Dec 1.
Published in final edited form as: J Thorac Cardiovasc Surg. 2021 Oct 13;164(6):e371–e384. doi: 10.1016/j.jtcvs.2021.07.062

Figure 4: EV-treatment reduced calcium mineralization and cardiomyocyte hypertrophy.

Figure 4:

Von Kossa counterstained with hematoxylin and eosin were used to measure (C) calcium deposition and (D) cardiomyocyte diameter. Five areas at 10x magnification per animal were measured and analyzed by 2-way ANOVA. Western blots of protein lysates from porcine ischemic myocardial tissue were performed as indicated with quantitative analyses normalized to α-tubulin and representative images (B) Osteopontin and (D) cardiac myosin. Blot for (B) is provided in supplementary Figure S1. Data is graphed as mean with SEM, with main effect of diet as large bracket and main effect of EVs as inner brackets. SPP1, osteopontin; β-MHC, heavy chain cardiac myosin. Scale bar are as follows (A) 100 μm and (B) 25 μm.