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. Author manuscript; available in PMC: 2023 Apr 30.
Published in final edited form as: J Mol Biol. 2021 Oct 13;434(8):167304. doi: 10.1016/j.jmb.2021.167304

Figure 1.

Figure 1.

(a) The scheme demonstrating the strategy to develop ATMW-BL21 strain from BL21(DE3). In the presence of the pUltra plasmid expressing the yeast tryptophanyl pair, EcTrpRS and tRNAEcTrp were replaced with zeocin and gentamycin resistance genes, respectively, by recombination. The plasmid encoding the recombination machinery, pKD46, was removed at the end by heat induction. (b) ATMW-BL21 exhibits a growth rate comparable to its progenitor BL21(DE3) strain, with or without the pUltraG complementation plasmid. OD600, optical density measured at 600 nm.