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a) Evaluating the incorporation of different tryptophan analogs into sfGFP-151-TGA in ATMW-BL21 using three different pEVOL vectors. EcTrpRS-h13 and –h14 show slightly different substrate preference. The use of the stronger leuV promoter to express the tRNAEcTrpUCA and tacI promoter to express the EcTrpRS provides substantial increase in suppression efficiency. (b) Structures of the tryptophan analog UAAs used in the study.
