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. 2022 Apr 12;13:1968. doi: 10.1038/s41467-022-29591-z

Fig. 7. CgRtt106 and CgSWI/SNF mediate antifungal resistance by upregulating expression of the multidrug transporter genes in C. glabrata.

Fig. 7

a RNA-seq analysis reveals reduction of mRNA of multidrug transporter genes CgCDR1 and CgCDR2 in the absence of CgRtt106 and CgSnf2 upon transient ketoconazole treatment in YEP. Data represents mean of three biological replicates. Significance determined by one-way ANOVA with post-hoc Tukey HSD test. ns, P > 0.05. b Positions of PDRE and PDRE-like motif in the CgCDR1 promoter. c, d ChIP-qPCR analyses of CgRtt106 (c) and the CgSwp82 SWI/SNF subunit (d) at the CgCDR1 promoter in YEP with and without ketoconazole. Proximal (blue) and distal (green) PDRE sites (illustrated in b) were analysed. Control locus (white) is coding region of the CgADY3 gene. Data represent mean of three biological replicates. Significance determined by one-way ANOVA with post-hoc Tukey HSD test. ns, P > 0.05. e Sensitivity to azole antifungal drugs of the C. glabrata reference strain (ATCC 2001) and C. glabrata lacking CgRtt106, CgSnf2 or CgCdr1. Five-fold dilutions of cell culture were spotted on YPD or YPD containing ketoconazole or fluconazole, and incubated at 30 °C for 2–3 days.