Pulsed-field gel electrophoresis of SmaI-digested total DNA from clinical S. aureus strains resistant to streptogramin A. (A) SmaI macrorestriction patterns; (B) hybridization patterns with the vga(A) probe (pIP1799) at high stringency (65°C, 5× SSPE buffer). Lanes 1, 8, and 9, bacteriophage lambda DNA concatemers (Bio-Rad); lanes 2, BM3385; lanes 3, BM3250; lanes 4, BM3252; lanes 5, BM3249; lanes 6, BM3327; lanes 7, NCTC8325 used as standard; lanes 10, BM3318.