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. 2022 Apr 12;13:1961. doi: 10.1038/s41467-022-29494-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a and b Transformation frequencies after electroporation with a single-stranded DNA (75-mer) coding for streptomycin resistance as donor DNA. Bars correspond to the average and standard deviation from the number of experiments indicated within the bars. * Assay detection limit as determined considering one colony obtained in a recA mutant. c Selective affinity of ComFC for single-stranded DNA. Indicated concentrations of His₆-ComFC were incubated with a Cy5-labelled single- or double-stranded DNA oligonucleotide. The nucleoprotein complexes were resolved by native-PAGE. d Protection against nuclease activity. 78 nt long fluorescent oligonucleotide was incubated 50 min with 5 units of Mung Bean Nuclease (MBN) after 20 min of preincubation with drSSB or ComFC at the indicated concentrations. Products were analysed on a native polyacrylamide gel. Blots are representative of two experiments. Source data are provided as a Source Data file.