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. 2022 Apr 12;12:6140. doi: 10.1038/s41598-022-09625-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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$D L F_{150}$ & $λ$ metrics show consistent and earlier trends compared with the tumour volume response, showing promise as candidate predictive biomarkers. The mean normalized $D L F_{150}$ (solid black, left y-axis) and λ (solid grey, right y-axis) for the $30$ Gy treated mice $(n = 6)$ seem to precede the temporal evolution of the mean normalized tumour volume (dotted black, left y-axis). Arrows represent approximate time-to-peak in response maxima and minima, respectively. Error bars represent standard deviations.