FIGURE 1.

Short-term dietary protein or carbohydrate restriction alters body composition. Male B6D2F1 mice were fed one of six diets for 8 days (n = 6 per diet) with varying macronutrient compositions as depicted in (A). The energy density of each diet is listed above the associated bar. (B) Changes in body weight after 8 days of diet shown as the percent of the initial weight. (C) Average energy intake across 8 days of diet feeding. Intake was normalized to the summed body weight of 2 mice co-housed per cage and presented per cage. Fat mass (D) and lean mass (E) quantified by EchoMRI at the end of the diet period. (F) Liver weights at sacrifice after 6 h fasting period. Statistical analysis was performed by one-way ANOVA with Tukey’s multiple comparison test (A–D). Different letters indicate significant differences (p < 0.05), with common letters indicating no significant difference between groups. Values are represented as mean ± SD.