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. 2022 Mar 30;13:870183. doi: 10.3389/fimmu.2022.870183

Figure 2.

Figure 2

GPR105−/− suppresses MSU-induced NETosis. (A) Primary neutrophils purified from WT and GPR105−/− rats were incubated with Veh or MSU (250 μg/ml) for 4 h (n = 3). (B) Representative images of extracellular DNA staining (SYTOX green) in primary neutrophils. (C) Levels of IL-8 and IL-1β in the MSU-treated neutrophil supernatant were measured by ELISA. All values are presented as the mean ± SD (### p < 0.001 versus corresponding WT+Veh group. *** p < 0.001 versus corresponding WT+MSU group, one-way ANOVA). (D) Representative images of NET formation (citrullinated H3, green; MPO, red) in primary neutrophils. Representative images of apoptosis cell (TUNEL, green) in primary neutrophils. (E) Representative images of apoptosis cell (TUNEL, green) in primary Neutrophils.