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. 2022 Mar 30;13:867195. doi: 10.3389/fimmu.2022.867195

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Copyright © 2022 Moraes, Trentini, Fousteris, Eto, Chudzinski-Tavassi, Leite and Kanno

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Vectors pAN71-ltak63-lysA(t) and pAN71-ltak63-lysA(c) used for the complementation of lysine auxotrophic strains. (A) In pAN71-ltak63-lysA(t) the lysA gene is in tandem with ltak63, including a Shine-Dalgarno sequence (SD) between the genes. (B) In pAN71-ltak63-lysA(c) the lysA gene is under the control of the pGrOEL promoter. The KanR site was truncated by restriction digestion with NsiI or ClaI, respectively and self- ligated. Both plasmids share the same features such as oriM and oriC, the pAN promoter and the ltak63 sequence.