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Expression of LTAK63 in complemented auxotrophic BCG. Western blot of total protein extracts of wild-type BCG (C-); rBCG-LTAK63 (C+) and complemented auxotrophic strains obtained by transformation of BCGΔlysA with pAN71-ltak63-lysA either in the tandem construct (t) or cassette (c). Western blots were probed with mouse anti-serum raised against rLTAK63 (1:1,000). Molecular weight markers (MW) are indicated and the expected molecular weight of LTAK63 is indicated by an arrow (~31 kDa).
