FIGURE 6.

Previously hospitalised subjects during acute infection showed higher TIGIT+ CD4+ T‐cell levels and lower polyfunctional S‐ and N‐specific T‐cell response than non‐hospitalised subjects 7 months after SARS‐CoV‐2 infection. (A) TIGIT expression in each CD4+ T‐cell subset in previously hospitalised and non‐hospitalised subjects 7 months after SARS‐CoV‐2 infection. (B) S‐specific CM CD4+ T‐cell levels of combinations only including TNF‐α+ cells for five (IFN‐γ, TNF‐α, IL‐2, CD107a and PRF) functions (left panel). S‐specific CM CD4 T‐cell polyfunctionality pie charts (right panel). (C) N‐specific EM CD4+ T‐cell levels of combinations only including IFN‐γ+ cells for five (IFN‐γ, TNF‐α, IL‐2, CD107a and PRF) functions (left panel). N‐specific EM CD4 T‐cell polyfunctionality pie charts (right panel). (D) N‐specific CM CD8+ T‐cell levels of combinations only including TNF‐α+ cells for five (IFN‐γ, TNF‐α, IL‐2, CD107a and PRF) functions (left panel). N‐specific CM CD8 T‐cell polyfunctionality pie charts (right panel). (E) N‐specific CM CD8+ T‐cell levels of cells producing IL‐2 (left panel). Representative dot plot showing IL‐2 production in N‐specific CM CD8+ T‐cells (right panel). (F) N‐specific EM CD8+ T‐cell levels of cells producing PRF (left panel). N‐specific EM CD8 T‐cell polyfunctionality pie charts (right panel). For all the pie charts, each sector represents the proportion of SARS‐CoV‐2‐specific T‐cells producing two (blue) and one (yellow) function. Arc represents the type of function (IFN‐γ, TNF‐α, IL‐2, CD107a and PRF) expressed in each sector. Permutation test, following the Spice version 6.0 software was used to assess differences between pie charts. Each dot represents an individual. ROUT method was utilised to identify and discard outliers. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Mann–Whitney U‐test was used for groups’ comparisons. (H, n = 19; NH, n = 14)