Figure 6.

Effect of Tecr knockdown on synthesis and transport of phospholipids in primary HUVECs. (a) The volcano plots visualized the different lipid molecules between control and Tecr^KD ECs. n = 6 per group. (b) The effect of Tecr on elongating FAs was analyzed by targeted lipidomic between control and Tecr^KD ECs. (c) The effect of Tecr on desaturating FAs was analyzed by targeted lipidomic between control and Tecr^KD ECs. ^∗∗∗∗p < 0.0001, ^∗p = 0.0264, unpaired t-test. (d, e) Total DHA levels (d) and total AA (e) levels in control and Tecr^KD ECs. DHA and AA levels were expressed as mean ± SEM of the percentage of total phospholipids. (d) p = 0.022; (e) p < 0.0001; unpaired t-test. Data are expressed as mean ± SEM.