. Author manuscript; available in PMC: 2022 Apr 13.

Published in final edited form as: Mol Reprod Dev. 2015 Oct 21;83(1):50–60. doi: 10.1002/mrd.22590

TABLE 1.

Blastocyst Development on Day 8 of Culture With Glucose (0, 1.5, or 4 mM) and/or DNP (0, 10, or 100 μM)

Number of blastocysts (%)

Glucose (mM)	DNP (μM)	Day 5 number treated	Pre-expanding and expanding blastocysts (n)	Expanded and hatched blastocysts (n)	Total (n)

0	0	400	51.7 ± 5.2 (103)^abcd	48.3 ± 5.2 (88)^abc	47.4 ± 2.8 (191)^ab
	10	325	48.1 ± 5.6 (78)^acd	51.9 ± 5.6 (94)^abc	50.9 ± 4.3 (172)^b
	100	350	57.1 ± 11.3 (55)^b	42.9 ± 11.3 (44)^abc	26.2 ± 4.2 (99)^c
1.5	0	350	60.7 ± 6.0 (158)^abc	39.3 ± 6.0 (102)^a	74.3 ± 2.2 (260)^d
	10	350	61.5 ± 5.3 (152)^abc	38.5 ± 5.3 (97)^a	71.1 ± 2.8 (249)^d
	100	350	65.2 ± 4.0 (165)^ab	34.8 ± 4.0 (82)^a	71.4 ± 3.5 (247)^d
4	0	325	38.2 ± 4.5 (52)^cd	61.8 ± 4.5 (91)^bc	44.4 ± 2.9 (143)^ab
	10	325	35.4 ± 5.9 (44) ^d	64.6 ± 5.9 (78)^b	34.7 ± 2.0 (123)^ac
	100	325	51.3 ± 7.3 (44)^abc	48.7 ± 7.3 (56)^ac	26.7 ± 4.9 (100)^c

Percentages development of pre-expanding and expanding, as well as expanded and hatched blastocyst, were calculated based on total blastocysts with in a treatment. Data were arcsine transformed prior to statistical analysis by GLM, with culture (n = 26) included as a random factor. Superscripts represent statistically significant differences between treatments within a developmental stage (P < 0.05). Data are presented as mean ± standard error of the mean.