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Kinetic parameters of the β-lactamase with the D90N mutation for cephalothin and its zinc content
| β-Lactamase | kcat (s−1) | Km (μM) | kcat/Km (μM s−1) | Zinc content (mol/mol of enzyme) |
|---|---|---|---|---|
| Wild type | 35 ± 2 | 2.7 ± 0.2 | 13.5 ± 0.4 | 2.00 ± 0.02 |
| D90N | 0.04 ± 0.001 | 2.81 ± 0.50 | 0.017 ± 0.003 | 2.29 ± 0.18 |
