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. 2022 Feb 16;219(5):e20210562. doi: 10.1084/jem.20210562

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© 2022 Mysore et al.

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Figure 4. — CCR2 on hematopoietic lineages and TNFR2 on parenchymal cells are required for the generation of immature macrophages and kidney injury. (A) NTN was induced in mice heterozygous for CCR2 and CX3CR1 (Het), Cx3cr1 KO, Ccr2 KO, or lacking both receptors (DKO) and analyzed along with Het untreated animals (−). On day 14, Ly6C and F4/80 were examined on kidney samples to distinguish P1–P4 monocyte-macrophage populations. (B) NTN was induced in radiation chimeric Wt recipients reconstituted with heterozygous, Cx3cr1 KO, Ccr2 KO, or DKO bone marrow. Representative FACS plots for indicated chimeric mice subjected to NTN and the frequency and absolute counts of P1–P4 populations are shown. (C) NTN was induced in radiation chimeras of Wt or Tnfr2 KO recipients reconstituted with bone marrow heterozygous for CCR2 and CX3CR1 and analyzed as in A. FACS plots and frequencies and absolute counts of P1–P4 populations are shown. (D) Proteinuria evaluated in radiation chimeras examined in B (left) and C (right). Two independent experiments were performed. *, P < 0.05; **, P < 0.01; ***, P < 0.005 (Tukey’s multiple comparison test).