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. 2022 Feb 16;219(5):e20210562. doi: 10.1084/jem.20210562

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© 2022 Mysore et al.

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Figure 6. — Neutrophil depletion attenuates the intravascular conversion of monocytes to immature macrophages. Mice were left untreated (−) or given the neutrophil-depleting antibody 1A8 before inducing NTN. 18 h after induction of NTN, anti-CD45 mAb was injected i.v. 3 min before harvesting the blood and kidney. Total neutrophil and monocyte counts (A and C) and the frequency and number of Ly6C⁺ monocytes expressing MHCII and/or F4/80 (B and D) in the blood (A and B) and kidney (C and D) are given. Data are from a single experiment with n = 5 individual mice per group. Statistical significance was determined by two-tailed Mann–Whitney U test. *, P < 0.05; **, P < 0.01; ***, P < 0.005.