Figure S2.

NTN-induced monocyte recruitment per se is not affected, but proteinuria and histological evidence of renal injury are reduced in CCR2-deficient mice. (A) The number of CD11b⁺Ly6C⁺ and CD11b⁺Ly6C⁻ cells in the kidney and blood of Wt, Cx3cr1 KO, and Ccr2 KO mice 60 min after NTS injection (in the absence of priming with CFA). As CCR2 KO mice had a reduction in circulating blood monocytes, a ratio of monocytes in kidney vs. blood was calculated as an index of monocyte recruitment. (B) NTN was induced in Wt, Cx3cr1 KO, and Ccr2 KO mice and analyzed along with untreated Wt (−) animals. Representative FACS plots using Ly6C and MHCII to distinguish mononuclear populations P1–P4 and frequencies in each population are shown. (C) Proteinuria in radiation chimeras of Wt recipients reconstituted with Het (Ccr2^rfp/+Cx3cr1^gfp/+, n = 8), Cx3cr1 KO (Cx3cr1^gfp/gfp, n = 8), Ccr2 KO (Ccr2^rfp/rfp, n = 5), or DKO (Ccr2^rfp/rfpCx3cr1^gfp/gfp, n = 8) bone marrow on day 14 after NTN induction. Histological scores for glomerular, interstitial, and tubular injury in Het (n = 4), Cx3cr1 KO (n = 4), Ccr2 KO (n = 5), and DKO (n = 7) on day 14 after NTN induction. Two independent experiments were performed. *, P < 0.05, **, P < 0.01, ***, P < 0.005 (Tukey’s multiple comparison test).