Figure S4.

scRNA-seq characterizes monocytes in contact with TNF-activated endothelial cells. (A) UMAP showing the distribution of monocytes which are Wt and Ccr2 KO cultured with TNF-activated endothelial cells (EC) or medium (Med) alone (n = 5,608 cells in total). (B) Heatmap of all markers of identified monocyte clusters. The number of UMIs as the expression of markers is shown. (C) Gene set enrichment in GO terms related to biological processes (GSEA-GO-Bp) for the genes overexpressed in Wt monocytes cultured with activated endothelial cells in comparison to monocytes cultured in medium alone (Wt:EC vs. Wt:Med), Ccr2 KO monocytes cultured with endothelial cells in comparison to monocytes cultured in medium alone (Ccr2 KO:EC vs. Wt:Med), and Wt monocytes in comparison to Ccr2 KO monocytes cultured with activated endothelial cells (Wt:EC vs. Ccr2 KO:EC). OR, odds ratio.