Extended Data Figure 8 (related to Figure 3). Extended analysis of genes distinguishing between branches in URD tree.

a Feature importance (0.5 power transformed – dot size) and average expression of genes predicted to be involved in cell types divergence (row-scaled – color). Top 10 genes per branch, ranked by their Friedman MSE score (importance) for distinguishing between cells in one branch versus cells in sibling and parent branch. Color bar at top indicates branch-points marked on the tree to the left. Arrows indicate daughter branches. Genes in red correspond to transcription factors. Expression in parent branch not shown. b Gene Ontology analysis showing molecular function enrichment among genes involved in branch-points as determined in panel a. c Simplified URD branching trees on which average gene expression within a segment and a pseudotime bin is overlaid on the tree structure, showing restricted expression patterns of genes identified in a.