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. Author manuscript; available in PMC: 2022 Apr 13.
Published in final edited form as: Nature. 2021 Jun 23;595(7868):554–559. doi: 10.1038/s41586-021-03670-5

Figure 5. Fezf2 prevents acquisition of callosal identity in CFuPN.

Figure 5

a Gene programs of connected modules (as in Fig. 3c), colored by Fezf2 score.

b Expression of affected modules in Fezf2 KO E15.5 cortex. Average expression of the top 30 genes of the SCPN and CThPN modules, in AP, IP and excitatory neurons (N), by genotype. Differential expression between control (Het) and KO neurons (two-sided Wilcoxon Rank Sum test, Bonferroni correction).

c-d UMAP visualization of scRNA-seq from E15.5 control and KO cortices, by genotype (left) and cell type (right). Proportion of cell types by genotype (bottom).

e A classifier trained on the wild-type atlas, mostly assigned KO-specific clusters (red in UMAP insets) to CThPN and layer 5&6 CPN.