Table 1: E5-ILP medium composition.
Pipette all the reagents except the chemically defined lipids and anti-mycoplasma reagent directly into the upper chamber of a 0.22 μm Stericup filtration unit. After filtration, add the lipids and anti-mycoplasma reagent. Store at 4 °C for up to two weeks.
| Reagent | Stock conc. | Working conc. | Amount per 250 mL |
|---|---|---|---|
| TeSR-E5 | n/a | n/a | 238.48 mL |
| PVA | 10% | 0.25% | 6.25 mL |
| Pen-Strep | 100x | 1x | 2.5 mL |
| ITSE | 100x | 0.1x | 250 μL |
| Chemically defined Lipids | 100x | 1x | 2.5 mL |
| Plasmocin | 25 mg/mL | 2.5 μg/mL | 25 μL |