Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Pipette all the reagents except and anti-mycoplasma reagent directly into the upper chamber of a 0.22 μm Stericup filtration unit. Once filtered, add anti-mycoplasma reagent. Store at 4 °C for up to two weeks.
| Reagent | Stock conc. | Working conc. | Amount per 500 mL |
|---|---|---|---|
| DMEM (Low Glucose) | n/a | n/a | 417.5 mL |
| KOSR | n/a | 10% | 50 mL |
| PVA | 10% | 0.25% | 12.5 mL |
| Pen-Strep | 100x | 1x | 5 mL |
| MEM-NEAA | 100x | 1x | 5 mL |
| GlutaMAX | 100x | 1x | 5 mL |
| HEPES | 100x | 1x | 5 mL |
| Plasmocin | 25 mg/mL | 2.5 μg/mL | 50 μL |
