FIGURE 3.
Aged islets have increased in p27KIP1 protein levels as compared to young islets by immunofluorescence assay. No significant difference was observed between young (5 week) and aged (5 month) islet protein levels of p21CIP1 (A-B) or p57KIP2 (E-F). However, p27KIP1 (C-D) protein levels were significantly increased in aged islets as measured by immunofluorescence. (G) Percent p27KIP1 is significantly greater in aged islets, regardless of islet size, with comparison of intercepts. n = 5 mice per age, minimum of three sections per rat. ****p < 0.0001. All images have a set scale bar of 60 μm