Table 1.

Antibody Fc domain amino acid sequences

Residue #	233	234	235	236	237	267	268	296	298	327
Wild-type	E	L	L	G	G	S	H	Y	S	A
Library	H Q D E	E D Q H V L	E D Q H V L	E D G	E D G	E D K G N R S	D S Y A P H	H Y	H N R S	E D A
3A	D	V	V	G	G	E	D	H	S	A
3E	D	V	V	G	G	D	D	Y	R	A
3F	D	V	D	G	G	G	D	H	S	A
4A	D	V	D	G	G	E	D	H	S	A
Acid-Fc (3A2/4A2)	E	L	L	G	G	E	D	H	S	A
3E2	E	L	L	G	G	D	D	Y	R	A
3F2	E	L	L	G	G	G	D	H	S	A

Shown are the sequences for wild-type (WT) human IgG1 Fc, the residues allowed at each targeted position during library design and the changes present in selected Fc variants. Wild-type residues are represented in regular font, while introduced changes are shown in bold.