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. 2022 Mar 10;298(4):101819. doi: 10.1016/j.jbc.2022.101819

Figure 3.

Figure 3

Enrichment of State 2 using PGT151 Fab causes a minor to moderate reduction in binding of State 1- and State 3-preferring antibodies. A, binding of serially diluted HIV-1–specific bnAb IgGs (various colors) to 293T cell surface–expressed, fully cleaved JR-FL Env trimers pretreated with PGT151 Fab (solid lines) or with PBS (dotted lines) are shown. The asterisk indicates that IgG binding was competed by presence of the indicated Fab used for trimer pretreatment. B, ratios of area under the curve of PGT151-treated trimers (AUCTx) to area under the curve of mock-treated trimers (AUCPBS) are plotted for each bnAb. Shown are mean ± SD of at least two experiments. At right are shown example ABR calculations from a representative experiment. ABR, antibody binding ratio; bnAb, broadly neutralizing antibody; Fab, fragment antigen binding.