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. 2022 Mar 11;298(4):101821. doi: 10.1016/j.jbc.2022.101821

Figure 2.

Figure 2

Gal-9 mAbs protect T cells from Gal-9-induced death. A, gating strategy to quantify viable CD4+ and CD8+ T cells by flow cytometry. Pseudocolor plots of events equivalent to 3000 counting beads. B, analysis of the survival of CD4+ and CD8+ T cells by flow cytometry. T cells were incubated with 2 μg/ml Gal-9 in the presence of indicated doses of control mouse IgG or different Gal-9 mAb clones. After overnight incubation, CD4+ and CD8+ T-cell survival was measured as described for (A). C, quantitation of the survival of CD4+ and CD8+ T cells. The experiment was performed as described for (B). CD, cluster of differentiation; Gal-9, galectin-9; IgG, immunoglobulin G; mAb, monoclonal antibody.