Fig. 3.

(A) Background-subtracted sensorgrams corresponding to assays of multiple samples containing same (CH II and CH III) and different (CH IV and CH V) antibody concentrations. The black, red, blue, and green sensorgrams represent signals obtained from CHs II, III, IV, and V. The dashed line is the threshold at which the next channel should be open for further measurements. The vertical arrows indicate the opening of each channel. The antibody molecules captured in the four channels were simultaneously removed with a single injection of 20 mM NaOH (the inverted peak after the dashed arrow). The flow rate was 80 μL min⁻¹. (B) The sensorgram of the first eleven injections (solid black) of 8.0-μg mL⁻¹ antibody shown in Fig. 3A, overlaid with a replicate performed after regeneration of CH II (dotted red). (C) The sensorgram of the first six injections of 16.0-μg mL⁻¹ antibody solution into CH III, as shown in Fig. 3A. (D) The sensorgram showing two replicate injections of five different antibody concentrations (4.0, 4.0, 10.0, 10.0, 16.0, 16.0, 8.0, 8.0, 2.0 and 2.0 μg mL⁻¹) below the threshold in CH IV and an injection of 12.0 μg mL⁻¹ antibody solution afterwards. (E) The sensorgram (solid black) shows the injections of randomized antibody concentrations (6.0, 2.0, 10.0, 16.0, 8.0, 14.0, 16.0, 8.0, and 4.0 μg mL⁻¹) into CH V as in Fig. 3A, and the dotted red sensorgram denotes six injections of 16.0 μg mL⁻¹ into this channel after sensor regeneration. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)