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. 2022 Mar 30;13:834776. doi: 10.3389/fmicb.2022.834776

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Copyright © 2022 Robeson, Manna, Randolph, Byrum and Hakkak.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Robust Aitchison PCA plot of metagenome samples processed through MetaPhlAn 3 and DEICODE and visualized with qurro. Bacterial species not present in at least 50% of samples were removed from the analysis. Separation of Control and Metformin treated groups was significant (p-value 0.002). (B) Corresponding log-ratio rankings for individual bacterial taxa oriented to Axis 1 (right). Numerator (red) / Denominator (blue) ratios of these taxa were computed to generate a box-whisker plot (left), p-value 0.0025. These results were corroborated with songbird analyses.