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. 2022 Mar 30;13:834776. doi: 10.3389/fmicb.2022.834776

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Copyright © 2022 Robeson, Manna, Randolph, Byrum and Hakkak.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Robust Aitchison PCA plot of metagenome samples processed through HUMAnN and DEICODE and visualized with qurro. Pathways not present in at least 50% of samples were removed from the analysis. These values Separation of Control and Metformin treated groups was significant (p-value 0.012). (B) Corresponding log-ratio rankings for Pathways oriented to Axis 1 (right), displaying only pathways contributed by Bacteroides spp. Numerator (red)/Denominator (blue) ratios of these pathways were computed to generate a box-whisker plot (left), p-value 0.008. These results were corroborated with songbird analyses.