Fig. 5.

Cardiac-Specific overexpression of Mfn2 promoted mitochondrial fusion and improved cardiac function in the Dox-treated mice. (A) Representative blots and quantitative data of Mfn2. The blotting experiments were conducted 3 times independently per group in Figure A. (B) Representative images of M-mode echocardiography (Quantitative data are presented in C-D). (C) EF, ejection fraction. (D) LVESV, left ventricular end-systolic volume. (E) Serum cTnT level measured by ELISA. (F) Representative mitochondrial images obtained by transmission electron microscope (Quantitative data are presented in G-I). Original magnification × 15000. Representative mitochondria on the bottom images (local magnifications of top images) were marked with light green pseudo-color to highlight the changes of the mitochondria. (G) The proportion of mitochondria in a specific area categorized into three size groups (< 0.6 μm², within 0.6–1.0 μm², >1.0 μm²) was counted. (H) Quantitative data of mean mitochondrial size. (I) Quantitative data of mitochondrial number per μm². n = 6 independent experiments per group in Figure B–I. One-way ANOVA with Turkey's multiple comparison test was used. **P < 0.01, ***P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)