Fig. 4.

CD8⁺and CD4⁺Teff surface proteomes respond similarly to hypoxia.A, CD4⁺CD25^- T cells were stimulated with anti-CD3/anti-CD28 beads in either normoxic (20% O₂) or hypoxic (1% O₂) conditions for 3 days. Volcano plot shows compiled results from N = 3 donors. Proteins with a −/+1.5-fold change and p < 0.05 were included in downstream analysis. Proteins significantly down- (blue) or upregulated (red) are indicated. B, Spearman correlation comparing the log₂(Enrichment Ratio) for both CD8⁺ and CD4⁺ T cells activated under hypoxic conditions. Venn diagrams showing proteins commonly down- (C) or upregulated (D) in hypoxia on both CD8⁺ and CD4⁺ cells. Below each Venn diagram are results from a GO biological process pathway analysis for commonly regulated proteins using the STRING database. The number of proteins identified and analysis FDR for each process are indicated.