Figure 5.

HPLC analysis of PHB accumulated in H. elongata wildtype in low salt condition (0.17 M NaCl) vs. high salt condition (2 M NaCl). At a retention time of approximately 3.25 min a distinct peak is visible for all three replicates grown at low salt while at high salt no peak can be observed. Analysis was performed by conversion of PHB into crotonic acid and detection at 210 nm.