FIG 3.

Development of murine polymerase I-mediated HRTV reverse genetics system. (A) Diagram of the production of HRTV using a reverse genetics system. BSR T7/5 cells were transfected with murine polymerase I-driven plasmids to generate S, M, and L viral genomes and helper plasmids to supply viral N and L proteins to the cells. Recombinant viruses were generated in BSR T7/5 cells. (B) Comparison of replication kinetics of wtHRTV and rwtHRTV in Vero 9013 cells. Results are expressed as the mean viral titer of triplicate experiments. Error bars denote the SD. ***, P < 0.001; *, P < 0.05; ns, P > 0.05. Experiments shown in B were performed in triplicate wells for each condition and repeated three times.