FIG 5.
Comparison of the viral RNA loads in the plasma and each organ of mice infected with rwtHRTV or rHRTV-NSsKO. AG129 mice (n = 5/group) were infected with 1.0 × 102 FFU of rwtHRTV or rHRTV-NSsKO. On 7 dpi, mice were euthanized, and plasma and organ samples were collected. (A and B) RNAs were extracted, and viral RNA loads in plasma and organs were determined using qRT-PCR. (C, D, and E) Hematoxylin and eosin (H&E) staining and IHC analysis using an anti-N antibody were performed on spleen (C), liver (D), and kidney samples (E). UDL, under detection limit. Error bars in A and B represent the SEM. **, P < 0.01. Dashed lines in B represent detection limits. The scale bars in C, D, and E represent 50 μm.