FIG 2.

Low pH enhances AiV capsid alterations. AiV analyzed by PaSTRy over a range of pH values (pH 7.0, 6.2, 5.9, 5.6, 5.0, and 4.9) with incremental increases of temperature of 1°C every 30 s. Experiments were either put in at room temperature (A and D) or heated at either 56°C (B and E) or 59°C (C and F) for 10 min and then chilled on ice for 2 min prior to being put on the Stratagene MX3005p quantitative PCR (qPCR) system. (A to C) Relative fluorescence of SYTO9 nucleic acid-binding dye, where exposure is inferred from increasing signal of viral RNA. (D to F) Relative fluorescence of SYPRO orange hydrophobic protein residue-binding dye, where increasing signal infers exposure of hydrophobic capsid components. All results are normalized to maximum signal for each experiment, representing 100% signal. All experiments were performed in triplicate; this is a representative trace.