Alignments of VP4- and VP0-encoding picornavirus sequences. Alignments of picornavirus VP4 sequences (A) and VP0 sequences (B) were carried out using the MUSCLE sequence alignment tool (55). The amino acids are colored using the Zappo color scheme as follows: aliphatic/hydrophobic (pale pink), aromatic (orange), positively charged (purple), negatively charged (red), hydrophilic (green), conformationally special (magenta), and cysteine residues (yellow). Peptide sequence regions for AiV, LV, and RV are highlighted with a black box.