Antisense RNA transcripts of HIV in MDM and CD4 infection. (A) Schematic of HIV genome showing the position of three HIV-specific antisense reverse transcription primers (AS RT1, AS RT2, or AS RT3) and four qPCR primer pairs (1, 2, 3, and 4) used for antisense transcript quantification. (B) RT-qPCR of antisense transcripts in uninfected and YU2-infected CD4 T cells and MDMs, calculated relative to cellular GUSB sense transcript. (C) RT-qPCR of antisense transcripts in YU2-infected CD4 T cells and MDMs with or without addition of RT. (D) Sense transcription of nef or tat for the same infection and RNA samples as shown in panel B.