FIG 7.
CD4- and CD4mc-mediated activation of infection of CD4-negative cells by HIV-1 Env variants. Wild-type HIV-1AD8 (A) and 2-4 RM6 AE viruses (B) containing the indicated MPER changes were spinoculated on CD4-negative, CCR5-expressing Cf2Th-CCR5 cells, after which serial dilutions of sCD4-Ig or BNM-III-170, a CD4-mimetic compound, were added. Note that a lower range of sCD4-Ig and BNM-III-170 was used in the bottom section of (A). Forty-eight hours later, the cells were lysed, and luciferase activity was measured. The results shown are representative of those obtained in two independent experiments, expressed as means and standard deviations from triplicate luciferase readings.