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. 2022 Mar 30;9:858603. doi: 10.3389/fnut.2022.858603

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Copyright © 2022 Zhang, Wu, Zhao, Yang, Chen, Wang, Wang and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MS imaging-based visual mapping profiles of BAs distributed within regions of colon. MS ion images representing spatial distributions of NPA (m/z 290.0823 ± 0.05) and the identified BAs at CDCA/UDCA/DCA m/z 391.2854 ± 0.05 (A), TCDCA/TUDCA/TDCA m/z 498.2895 ± 0.05 (B), LCA m/z 375.2905 ± 0.05 (C), TLCA m/z 482.2946 ± 0.05 (D), TCA/TMCA m/z 514.2844 ± 0.05 (E) and GCA m/z 464.3018 ± 0.05 (F) for the whole section of mouse colon (Scale bars: 700 μm). Overlay: Overlay of each MS ion image and optical image. All ion images were normalized to the 9AA matrix signal. Abbreviation: CD, control diet group; HFD, high-fat diet group.