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. 2022 Mar 30;9:858603. doi: 10.3389/fnut.2022.858603

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Copyright © 2022 Zhang, Wu, Zhao, Yang, Chen, Wang, Wang and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Distribution profiles of BAs in colon were studied through comparison of relative intensities of BAs by the values of p between ROI-1 and ROI-2. Signal intensities of targeted BAs in ROI of two different regions of colon were depicted and signal normalization was performed by pixel. ROI-1: submucosa, muscular layer and adventitia; ROI-2: mucosa layer. The letter ‘p’ denotes the statistical p-value of the comparison, significant differences were highlighted in green font while insignificant differences were highlighted in red font (A). The spectrum of each ROI which set in (A). The spectra were vertically inverted, with that of ROI-1 above in red and that of ROI-2 below in blue (B).