Chromatin tracing and Multiplexed Imaging of Nucleome Architectures (MINA) in single mammalian cells and tissue

. Author manuscript; available in PMC: 2022 Apr 13.

Published in final edited form as: Nat Protoc. 2021 Apr 26;16(5):2667–2697. doi: 10.1038/s41596-021-00518-0

Reagent	Quantity (μL)
DEPC-treated ddH₂O	6.16
CTP mix	1.34
NTP mix	10
T7 RNA polymerase mix	2
RNasin Ribonuclease Inhibitor	1
Purified DNA from step 15	10
Total	30.5