| Reagent | Quantity (μL) |
|---|---|
| dNTP mix (10 mM) | 12 |
| Reverse transcription primer (100 μM) | 10 |
| Maxima H Minus Reverse Transcriptase | 1.5 |
| 5x reverse transcription buffer | 15 |
| RNasin Ribonuclease Inhibitor | 1.5 |
| Unpurified RNA product from step 16 | 30 |
| Total | 70 |
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